This facility assembles different approaches to study metabolism in cell-free and cellular systems using HPLC and (spectro)photometric analysis, as well as respiration, heart function, and hypoxia.


Quantitative in vitro assays

based on fluorescence, luminescence or visible light detection/absorbance, eventually coupled to upstream separation by HPLC (enzyme activities, markers of protein and lipid oxidation, specific metabolites like adenylates, (phospho)creatine and antioxidants, mitochondrial calcium retention capacity, etc.)


to quantify mitochondrial respiration of intact cells, permeabilized cells or isolated mitochondria

Perfusion of heart in situ

recording of performance parameters

Multi-scale analysis (proteins, cells, organs, rodents) of hypoxia together with other facilitie