Metabolic analysis & Respirometry

Quantitative in vitro assays

based on fluorescence, luminescence or visible light detection/absorbance, eventually coupled to upstream separation by HPLC (enzyme activities, markers of protein and lipid oxidation, specific metabolites like adenylates, (phospho)creatine and antioxidants, mitochondrial calcium retention capacity, etc.)

Respirometry/oxygraphy

to quantify mitochondrial respiration of intact cells, permeabilized cells or isolated mitochondria

Perfusion of heart in situ

recording of performance parameters

Multi-scale analysis (proteins, cells, organs, rodents) of hypoxia together with other facilitie