Séminaire

AMP-activated protein kinase – from a metabolic signaling hub to a synthetic nanosensor of cell energetics

on the 19 November 2021
8h
DANS LE CADRE DE L’UE « MÉTHODES ET MODÈLES EN PHYSIOLOGIE ET PHARMACOLOGIE CARDIOVASCULAIRES ET EN NUTRITION »

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Uwe SCHLATTNER PU

LBFA Inserm U1055, Université Grenoble Alpes, Grenoble

« AMP-activated protein kinase – from a metabolic signaling hub to a synthetic nanosensor of cell energetics»
Availability of energy is a basic prerequisite for life. The energy flow through cells is central to all fundamental biological processes and its dysregulation is a common denominator of otherwise unrelated human pathologies, including chronic diseases linked to modern life style like diabetes. A basic requirement for cell survival and functional metabolism is maintenance of energy homeostasis, i.e. a stable energy state given by ATP/ADP and ATP/AMP ratios. The central sensor and regulator of energy state at the cellular level is the AMP-activated protein kinase (AMPK). Increasing AMP and ADP concentrations trigger an activating conformational switch within the heterotrimeric AMPK complex. Activated AMPK then initiates compensatory measures to increase ATP generation and to decrease non-essential ATP-consuming pathways. We have exploited the sensing properties of AMPK for the construction of a synthetic nanosensor of cellular energetics, AMPfret. Based on engineered AMPK fused to fluorescent proteins, AMPfret allows a direct, real-time readout of AMPK activation in space and time and at a single cell level via fluorescence resonance energy transfer (FRET). Such genetically encoded FRET sensors hold the promise of being game-changers in our understanding of cell energetics.

Location

La Tronche
Secteur santé
Amphi supérieur Sud
50 places disponibles
En raison des mesures sanitaires, ce séminaire est exclusivement réservé aux étudiants et personnels UGA
Mis à jour le 8 November 2021